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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What type of analysis can be performed using the rhAmpSeq™ CRISPR Analysis Tool?

The rhAmpSeq CRISPR Analysis Tool is capable of singleplex and multiplex analyses.

In singleplex analysis, the sample library has only one amplicon that is used to assess the editing of a single site in a sample. You would use singleplex analysis to screen numerous guide RNAs for on-target activity.

In multiplex analysis, the sample library is generated by multiplex PCR and is composed of multiple amplicons that assess the editing on multiple sites in a sample. You would use multiplex analysis to assess the on- and off-target editing of a guide RNA.