Your product is now available from Integrated DNA Technologies.
Many of the Swift products you have grown to love are now part of our new complete portfolio, xGen™ NGS. Through this new partnership we are pleased to offer you comprehensive next generation sequencing solutions.
xGen NGS—made for you.
Unsure of what products are available? Or, perhaps you’d like guidance on which products are compatible? If so, try our xGen NGS Solutions Builder Tool today.
Choose your region, country/territory, and preferred language
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Search all FAQs:
What secondary structure considerations need to be included when designing primers for PCR?
When designing primers for PCR, two types of secondary structures should be analyzed: dimers and hairpins. For dimers, both self- and hetero-dimers should be reviewed. In general, the ΔG value for dimer analysis should be between 0 to −9 kcal/mol. Values more negative than this may adversely affect PCR reactions. For hairpins, the Tm of the hairpin should be lower than the annealing temperature for the reaction.
The Tm for the strongest hairpin should be at least 50°C below the annealing temperature. These secondary structures and their corresponding stabilities can be calculated online with the IDT SciTools™ OligoAnalyzer Tool.