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Frequently asked questions

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What DsiRNA concentration do I need to use in my experiment to see knockdown?

Q: What DsiRNA concentration do I need to use in my experiment to see knockdown?

For an RNAi or antisense experiment , the actual level of target gene knockdown is related to the transfection efficiency. A positive control such as HPRT DsiRNA should be used in each experiment to assess transfection efficiency. In addition, IDT recommends using a dose-response curve of 0.1, 1, and 10 nM to determine maximum response.

For an RNAi or antisense experiment, the actual level of target gene knockdown is related to the transfection efficiency. A positive control such as HPRT DsiRNA should be used in each experiment to assess transfection efficiency.

In addition, IDT recommends using a dose-response curve of 0.1, 1, and 10 nM to determine maximum response.