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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Is library amplification required when using the Normalase™ Module?

Yes, libraries must be amplified and conditioned with Normalase™ primers. Even if library yields have met the 6 nM or 12 nM minimum threshold before PCR amplification, a minimum of 3 cycles is required to condition the library for downstream enzymology. The Normalase Module allows you to maintain your conventional library amplification reagents, with the exception of any amplification primers.

For indexing by ligation, use Reagent R5 for Normalase PCR in place of conventional amplification primers and see Appendix Section C in the protocol for recommended minimum PCR cycles.

For indexing by Normalase PCR, use Reagent R6 for Normalase CDA or Reagent R7 for Normalase UDI. Normalase indexing primers complete the adapter sequence as well as amplify and condition libraries for downstream Normalase steps. See Appendix Section C for minimum cycling as the use of Normalase indexing primers requires more cycles compared to conventional indexing primers.

Note: Refer to the xGen Normalase Module Protocol and the specific xGen™ library preparation protocols for minimum cycle recommendations.

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