Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
How do I incorporate a mutation into my donor template using the HDR Design Tool?
A mutation, such as an insertion, deletion, single nucleotide polymorphism (SNP), or multiple nucleotide polymorphism (MNP), can be incorporated in 2 different ways:
Enter the sequence to be inserted into the Mutation text box, and this sequence will be incorporated at the location marked by the 2 sliders.
Alternatively, move the sliders apart, and click on the bases between the sliders to change the sequence of each base, as needed.