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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Can I generate RNA libraries of different size ranges using xGen™ Broad-Range RNA Library Prep Kit?

Yes.

Please refer to the Appendix section of the xGen™ Broad Range RNA Library Prep Kit Protocol pertaining to expected results with alternate fragmentation times.

You can also refer to Appendix E for detailed instructions about fragmentation times and bead cleanup ratios required for different intended mean library sizes ranging from 380−480 bp (mean insert sizes ranging from 250−350 bp).



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