It is not recommended.
Fragmentation solely by bisulfite conversion results in a broad size distribution of larger DNA fragments. Such fragments may be converted into library molecules; however, the wide distribution of library molecules will cluster poorly on the flow cell. If skipping fragmentation is unavoidable, we recommend examining the size of library molecules prior to library quantification, and, if necessary, performing a right-side size selection to remove very large library molecules to improve clustering on the flow cell.
This scenario will result in reduced complexity of sample representation.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.