Convenient mismatch endonuclease assays that are based on analysis of PCR products spanning your region of interest
For estimating on-target editing efficiency or assessing known off-target effects in your CRISPR experiments, we recommend using the Alt-R Genome Editing Detection Kit (also compatible with ZNF- and TALEN-driven genome editing systems). This T7 endonuclease
I mismatch cleavage assay is ideal for directly analyzing your reaction products to detect multiple-base insertions and deletions.
The Surveyor Mutation Detection Kit allows simple, robust detection of SNPs. The key component, Surveyor Nuclease, cleaves mismatched DNA caused by single-nucleotide polymorphisms and small insertions or deletions.