Superior on-target coverage and uniformity
Figure 1. Highest on-target performance is achieved by xGen Exome Research Panel. Sequencing libraries were prepared using standard shearing and ligation-based library preparation, and then pooled into a 12-plex. The 12-plex pool was split into four 6 µg aliquots to assess each vendor’s exome panel. Target capture was performed according to each vendor’s recommended protocol. Enriched libraries were sequenced on a HiSeq® 2500 instrument (Illumina) in high output mode using 2 x 100 paired-end reads. On-target baes were determined with Picard (selected bases), using 35M reads per library.
Excellent coverage of RefSeq database with minimal sequencing
Figure 2. Most uniform coverage of exome achieved with xGen Exome Research Panel. (A) Coverage of exons 1 and 2 of RB1 shows that the xGen Exome Research Panel is the only panel to efficiently capture first exons. The xGen exome panel displays no decrease in first exon coverage with increasing sequencing depth. (B) The xGen Exome Research Panel provides the most complete end-to-end gene coverage using 100M reads.
Figure 3. Coverage profile of xGen Exome Research Panel most closely resembles whole genome data. A comparison of coverage depths of different exome panels to coverage depth from whole genome sequencing (WGS) of a matched library shows that the xGen Exome Research Panel provides the closest match to WGS. Analysis of GC content shows that the higher coverage of the xGen panel is driven by the more effective capture of GC content.