Lotus™ DNA Library Prep Kit: A single flexible workflow with endless applications

Since NGS is increasingly being adopted in clinical and research laboratories, scientists need a streamlined workflow that seamlessly transitions from library preparation to target enrichment, and enables multiple applications using a single library prep kit. The kit employs a combination of enzymatic fragmentation and unique end repair mechanisms in a single tube with an efficient adapter ligation strategy to generate high-quality NGS libraries in approximately 2 hours. The flexibility in the chemistry allows the user to pair any TA-ligation compatible adapter (full-length or stubby) with a sample indexing strategy of their choice for PCR-free, PCR-amplified, and targeted sequencing applications on Illumina platforms. This library prep kit is compatible with multiple custom IDT NGS adapters, including TruSeq™ (Illumina)-compatible Full-length Adapters and TruSeq-compatible Stubby Adapters and Indexing Primers, as well as our xGen™ suite of reagents for hybridization capture. We discuss how this workflow can be adopted for multiple applications including metagenomics, PCR-free whole genome sequencing, and whole exome sequencing.