Constraining Adaptive Mutations using Engineered Overlapping Sequences (CAMEOS) is a new computational platform that uses overlapping sequences to prevent protein function disruption. Read how Blazejewski, et al, used gBlocks Gene Fragments and tested their algorithm to ensure that synthetic protein encoding is safeguarded against mutations.
The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.
The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.
The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.
gBlocks Gene Fragments were used to generate the fatty acyl-CoA reductase gene from barn owl.
The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.
The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.
gBlocks Gene Fragments were used to generate mutant autonomously replicating sequences, or ARSs, associated with replication origins in yeast chromosomal DNA.
A gBlocks Gene Fragment was used to create an sgRNA expression vector for use with the CRISPR/Cas9 system.
gBlocks Gene Fragments were used to create sgRNA expression plasmids.
5 gBlocks Gene Fragments were used to assemble the ~2 kb coding sequence for Micalcl (NM_027587).
gBlocks Gene Fragments were used to generate codon-optimized cytochrome P450 enzymes, and other hemoproteins, in order to study their catalytic use in non-natural olefin cyclopropanation reactions.
gBlocks Gene Fragments were used to construct a codon optimized Cas9 sequence for expression in Drosophila. Optimization of the sequence was performed using the IDT Codon Optimization tool.
gBlocks Gene Fragments were used to create sgRNA, gene specific sequences.
gBlocks Gene Fragments were used to generate codon-optimized acRAF genes from <em>H. neapolitanus</em> and <em>P. marinus</em>.
gBlocks Gene Fragments were used as positive genotype controls for the described 5′ nuclease, SNP genotyping assay.
gBlocks Gene Fragments were used for creating sgRNA expression constructs for targeting mutant Cas9 variants to test either transcription activation or genome modification.
gBlocks Gene Fragments were used to create an Mxi1 transcription repressor domain and assembled with a dCas9 construct using the Gibson Assembly™ method into an expression vector. The resulting chimeric, transcriptional repressor was then shown to be targetable using the CRISPR/Cas9 mechanism.