BEGIN:VCALENDAR VERSION:2.0 METHOD:PUBLISH PRODID:-//Telerik Inc.//Sitefinity CMS 14.0//EN BEGIN:VEVENT DESCRIPTION:Confidence is the currency of science.\nIDT will be participati ng in NextGen Omics Series UK 2020 virtually this year. Learn about our so lutions for life sciences and medical research\, serving the academic\, cl inical\, biotechnology\, pharmaceutical development\, and agricultural res earch communities. IDT&rsquo\;s NGS products are a critical part of oncolo gy research\, inherited and infectious disease research\, biomarker develo pment\, diagnostics\, and early screening. The conference runs Nov. 4 thro ugh Nov. 6.\nWorkshop - on demand\nNov. 5\, 2020 | 10:00 &ndash\; 10:30 UT C+0\nA complete workflow for analysis of cfDNA: from plasma to variants\nP resented by: Nicole Roseman and Shilpa Parakh\n\n\n\n\nAs cost of sequenci ng continues to drop\, the throughput and complexity of NGS assays have ri sen precipitously. At the same time\, the types of samples being used for these assays have expanded as researchers find value in studying low input \, degraded biological samples\, such as cell free DNA (cfDNA). Obtaining actionable information from clinical samples is challenging due to loss du ring extraction\, low conversion during NGS library preparation\, drop in complexity during target enrichment\, and sequencing and PCR errors that l ead to low limits of detection. \n\nHere\, we present a complete workflow for effective analysis of <\;1% AF variants in cfDNA. Our magnetic bead- based extraction kit provides complex\, high yield recovery of cfDNA. A no vel library prep chemistry delivers higher complexity and coverage than co nventional TA ligation-based workflows\, enabling highly sensitive low-fre quency variant detection. In addition\, fixed unique molecular index (UMI) sequences aid strand-specific molecular indexing by independently tagging the top and bottom strands\, and can be used for various deduplication an d error correction strategies. \n\nOur target enrichment strategies mainta in high library diversity and on-target rates to enable low frequency vari ant calling regardless of panel size. We pair these chemistries with modul arly designed automation protocols which provide users with safe stopping points\, enabling customizable\, high throughput solutions. By combining a high conversion extraction and library preparation chemistry with efficie nt hybridization capture\, we demonstrate an effective approach to extract information from low quantity challenging samples.\n\n\nLearning objectiv es:\n1. Describe how to increase cfDNA extraction efficiency using magneti c bead&ndash\;based technology.\n2. Demonstrate how to maximize conversion \, coverage and complexity from low inputs of cfDNA during library prepara tion.\n3. Utilize fixed UMIs for bioinformatic error correction to reduce sequencing and PCR errors.\n4. Discuss how to increase throughput\, reduce hands-on time\, and minimize errors during complex NGS assays.\nNGS round table \;\nNov. 6\, 2020 | 16.30 \;&ndash\; \;17.00 UTC +0\nNG S with IDT. Prove it. We&rsquo\;ll help.\nHosted by: Nicole Roseman and Pe ter \;Verhasselt\n\n Benefits of liquid biopsy for cancer detection research\n What is DNA conversion and how you can achieve greater conv ersion\n How to obtain higher conversion and complexity using the xGen Prism DNA Library Prep kit to address the liquid biopsy sensitivity challe nge\n A description of unique molecular index (UMI) sequences and the b enefits of adding them during library prep for error correction\n\n\nExplo re our NGS solutions for precision health\nPortfolio overview\nBiomarker d iscovery\nxGen Prism DNA Library Prep Kit DTEND;VALUE=DATE:20201108 DTSTAMP:20240328T105137Z DTSTART;VALUE=DATE:20201104 LOCATION:Virtual SEQUENCE:0 SUMMARY:NextGen Omics Series UK UID:RFCALITEM638472198973175611 X-ALT-DESC;FMTTYPE=text/html:
IDT will be participating in NextG en Omics Series UK 2020 virtually this year. Learn about our solutions for life sciences and medical research\, serving the academic\, clinical\, bi otechnology\, pharmaceutical development\, and agricultural research commu nities. IDT&rsquo\;s NGS products are a critical part of oncology research \, inherited and infectious disease research\, biomarker development\, dia gnostics\, and early screening. The conference runs Nov. 4 through Nov. 6.
\nA complete workfl
ow for analysis of cfDNA: from plasma to variants
\nPresented by: Nic
ole Roseman and Shilpa Parakh
\n
\n\n
\nAs cost o
f sequencing continues to drop\, the throughput and complexity of NGS assa
ys have risen precipitously. At the same time\, the types of samples being
used for these assays have expanded as researchers find value in studying
low input\, degraded biological samples\, such as cell free DNA (cfDNA).
Obtaining actionable information from clinical samples is challenging due
to loss during extraction\, low conversion during NGS library preparation\
, drop in complexity during target enrichment\, and sequencing and PCR err
ors that lead to low limits of detection.
\n
\nHere\, we presen
t a complete workflow for effective analysis of <\;1% AF variants in cfD
NA. Our magnetic bead-based extraction kit provides complex\, high yield r
ecovery of cfDNA. A novel library prep chemistry delivers higher complexit
y and coverage than conventional TA ligation-based workflows\, enabling hi
ghly sensitive low-frequency variant detection. In addition\, fixed unique
molecular index (UMI) sequences aid strand-specific molecular indexing by
independently tagging the top and bottom strands\, and can be used for va
rious deduplication and error correction strategies.
\n
\nOur t
arget enrichment strategies maintain high library diversity and on-target
rates to enable low frequency variant calling regardless of panel size. We
pair these chemistries with modularly designed automation protocols which
provide users with safe stopping points\, enabling customizable\, high th
roughput solutions. By combining a high conversion extraction and library
preparation chemistry with efficient hybridization capture\, we demonstrat
e an effective approach to extract information from low quantity challengi
ng samples.
\n\n
\nLearning objectives:
\n
1. Describe how to increase cfDNA extraction efficiency using mag
netic bead&ndash\;based technology.
\n2. Demonstrate how to maximize
conversion\, coverage and complexity from low inputs of cfDNA during libra
ry preparation.
\n3. Utilize fixed UMIs for bioinformatic error corre
ction to reduce sequencing and PCR errors.
\n4. Discuss how to increa
se throughput\, reduce hands-on time\, and minimize errors during complex
NGS assays.
NGS with IDT. Prove it. We&rsquo\;ll help.
\nHosted by: Nico
le Roseman and Peter \;Verhasselt
\n<
strong>Explore our NGS solutions fo
r precision health
\nPortfolio overview
\n
Biomarker discovery
\nxGen Prism DNA Library Prep Kit
p>
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